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ORIGINAL ARTICLE
Year : 2023  |  Volume : 3  |  Issue : 1  |  Page : 2

High burden of extended spectrum β–lactamase (ESBL)–encoding genes in third–generation cephalosporin–resistant Escherichia coli recovered from frequently contacted surfaces and wastewater of selected healthcare institutions in Nigeria


1 Department of Microbiology, Olabisi Onabanjo University, Ago-Iwoye, Nigeria
2 Environmental Microbiology and Biotechnology Laboratory; Molecular Biology and Biotechnology Laboratory, Department of Microbiology, University of Ibadan, Ibadan, Nigeria

Correspondence Address:
Abimbola Olumide Adekanmbi
Environmental Microbiology and Biotechnology Laboratory; Molecular Biology and Biotechnology Laboratory, Department of Microbiology, University of Ibadan, Ibadan
Nigeria
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/2773-0344.363564

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Objective: This study aimed to determine the carriage of extended spectrum β-lactamase (ESBL) genes in third-generation cephalosporin-resistant (3GCR) Escherichia (E.) coli from frequently contacted surfaces, wastewater and disinfectant-cleaning solutions of selected healthcare institutions in South-western Nigeria. Methods: Samples were collected over three months for the isolation of 3GCR E. coli on MacConkey agar containing 6 μg/mL of cefotaxime. 3GCR E. coli isolates were identified by detection of uidA gene and susceptibility to selected antibiotics was performed using disc-diffusion method. Detection of ESBL genes was done using primer-specific PCR. Results: A total of 22 ESBL-producing E. coli (11 each from the frequently contacted surfaces and wastewater) were obtained from the pool of 3GCR isolates in this study. No isolate was recovered from the disinfectant-cleaning solution. All the ESBL-producing E. coli obtained from the frequently contacted surfaces and wastewater were multidrug resistant, with complete resistance observed to ampicillin, cefotaxime, cefpodoxime, tetracycline and ertapenem. The ESBL genotyping showed that 54.5% carried blaCTX-M, 63.6% carried blaTEM and 9.1% carried blaSHV in isolates from the frequently contacted surfaces, while 63.6%, 9.1% and 18.2% carried blaCTX-M, blaTEM and blaSHV, respectively, in the isolates obtained from the wastewater. Conclusions: This study showed a high burden of multidrug resistance E. coli on frequently contacted surfaces and wastewater of the studied healthcare institutions, indicating the need for good hygiene and proper mitigation measures to prevent potential public health and environmental challenges.


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